Depositor Sequences: None.
Addgene Sequences: Partial (1)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11529||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6194
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNAD kinase
Insert Size (bp)747
GenBank IDAAB91287 AF2373 NP_071196
/ Fusion Protein
- HisTag, TEV cleavable (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GTTGCATCACCTTCACCCTCTCC (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pLIC.B3.AF2373 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLIC.B3.AF2373 was a gift from Sung-Hou Kim (Addgene plasmid # 11529)
For your References section:Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP. Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH. J Mol Biol. 2005 Nov 25. 354(2):289-303. 10.1016/j.jmb.2005.09.026 PubMed 16242716
Generated by Addgene from plasmid data.