PurposeControl to test technique on a target that is known to work
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117801||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerKarl J. Clark
- Backbone size w/o insert (bp) 2329
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)4148
- Cloning method Gateway Cloning
- 5′ sequencing primer aaaagcaagaaagaaaactagagtgg
- 3′ sequencing primer atggctcataacaccccttg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Control integrates at the gene cx43.4 using the guide RNA Target "5'-GGGCCATATCTTGCCCACGA-3'" for wide-spread RFP expression in zebrafish embryos from 12hrs post injection through 5 days development
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Control-cx43.4-48H-pPRISM was a gift from Jeffrey Essner (Addgene plasmid # 117801 ; http://n2t.net/addgene:117801 ; RRID:Addgene_117801)
For your References section:Efficient targeted integration directed by short homology in zebrafish and mammalian cells. Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner J. Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968. 10.7554/eLife.53968 PubMed 32412410