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pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP
(Plasmid #92388)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92388 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTol2pA2
  • Backbone manufacturer
    Tol2kit
  • Backbone size w/o insert (bp) 5800
  • Modifications to backbone
    cryaa:RFP inserted
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ANNEXINV
  • Alt name
    ANXA5
  • Alt name
    annexin A5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5000
  • Entrez Gene
    ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)
  • Promoter ubb
  • Tag / Fusion Protein
    • mVenus (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP was a gift from Nicholas Cole (Addgene plasmid # 92388 ; http://n2t.net/addgene:92388 ; RRID:Addgene_92388)
  • For your References section:

    In vivo characterization of microglial engulfment of dying neurons in the zebrafish spinal cord. Morsch M, Radford R, Lee A, Don EK, Badrock AP, Hall TE, Cole NJ, Chung R. Front Cell Neurosci. 2015 Aug 31;9:321. doi: 10.3389/fncel.2015.00321. eCollection 2015. 10.3389/fncel.2015.00321 PubMed 26379496