pGEmCherry2
(Plasmid #118413)

• Purpose: Red fluorescent reporter for assessing CRISPR gRNA activity
• Depositing Lab: Mark Denham
• Depositing Organization: Aarhus University
  Located in Denmark
• Publication: Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. doi: 10.3389/fcell.2018.00054. eCollection 2018.

Backbone

• Vector backbone: pmCerulean3- N1
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

How to Design Genomic recognition sequence for GEmCherry2:
Genomic sequence require 20nt recognition sequence and PAM sequence.
In addition Oligos require overhangs to ligate into Xbal and BamHI sites.
Bottom strand requires BgIII site insead of BamHI to destroy site for subsequent restriction analysis. Inserted sequence may require additional nucleotide to be out of frame.

TOP Oligo 5` CTAGANNNNNNNNNNNNNNNNNNNN(PAM)C 3`
Xbal site 20nt sequence and PAM sequence and cytosine to destroy BamHI sequence.

BOTTOM Oligo 5'GATCG(MAP)NNNNNNNNNNNNNNNNNNNNT 3'

doi: 10.3389/fcell.2018.00054

DNA (Catalog # 118413-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 118413-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.