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pLenti-PGK-Neo-PIP-NLS-mVenus Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLenti-PGK-Neo-PIP-NLS-mVenus with the following enzymes: PvuI-HF and XhoI.

We expect the plasmid to give the following:
PvuI-HF: 3 bands: 6, 1.8, 1 kb.
XhoI: 2 bands: 7.5 and 1.5 kb.

The 3.5kb band appears to be contamination from the original entry vector.