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Addgene

pMP71-hPDL-1
(Plasmid #118631)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118631 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMP71
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6516
  • Modifications to backbone
    Gateway compatible (see Wälchli et al. 2011, PlosONE)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPDL-1
  • Alt name
    Programmed cell death 1 ligand 1
  • Alt name
    PDCD1 ligand 1
  • Alt name
    CD274
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    872
  • GenBank ID
    NM_001267706.1 NM_014143.3
  • Entrez Gene
    CD274 (a.k.a. B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1)
  • Promoter LTR

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was order as a synthetic fragment corresponding to the original coding sequence (isoform 1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP71-hPDL-1 was a gift from Sébastien Walchli (Addgene plasmid # 118631 ; http://n2t.net/addgene:118631 ; RRID:Addgene_118631)
  • For your References section:

    TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Function in B-cell Non-Hodgkin Lymphoma. Josefsson SE, Beiske K, Blaker YN, Forsund MS, Holte H, Ostenstad B, Kimby E, Koksal H, Walchli S, Bai B, Smeland EB, Levy R, Kolstad A, Huse K, Myklebust JH. Cancer Immunol Res. 2019 Mar;7(3):355-362. doi: 10.1158/2326-6066.CIR-18-0351. Epub 2019 Jan 18. 10.1158/2326-6066.CIR-18-0351 PubMed 30659053