pMP71-hPDL-1
(Plasmid #118631)

• Purpose: human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid
• Depositing Lab: Sébastien Walchli
• Publication: Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-362. doi: 10.1158/2326-6066.CIR-18-0351. Epub 2019 Jan 18.

Backbone

• Vector backbone: pMP71
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6516
• Modifications to backbone: Gateway compatible (see Wälchli et al. 2011, PlosONE)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hPDL-1
• Alt name: Programmed cell death 1 ligand 1
• Alt name: PDCD1 ligand 1
• Alt name: CD274
• Species: H. sapiens (human)
• Insert Size (bp): 872
• GenBank ID: NM_001267706.1 NM_014143.3
• Entrez Gene: CD274 (a.k.a. B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1)
• Promoter: LTR

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was order as a synthetic fragment corresponding to the original coding sequence (isoform 1)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry