PurposeBase editor made from APOBEC3G
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||119139||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerLiu Lab
- Backbone size w/o insert (bp) 7825
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameAPOBEC3G-Cas9 nickase-UGI
SpeciesH. sapiens (human)
Insert Size (bp)1152
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XmaI (not destroyed)
- 5′ sequencing primer CGTGTACGGTGGGAGGTCTA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:A3G-Cas9n-UGI was a gift from Reuben Harris (Addgene plasmid # 119139 ; http://n2t.net/addgene:119139 ; RRID:Addgene_119139)
For your References section:A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris RS. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. 10.1038/s41598-018-36739-9 PubMed 30679582