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A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris RS
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119129eGFP L202 ReporterActs as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.
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119130eGFP L138 ReporterActs as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.
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119132eGFP L202 gRNAgRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.
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119133eGFP L138 gRNAgRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.
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119135A3Bi-Cas9n-UGIBase editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction
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119136A3C-Cas9n-UGIBase editor made from APOBEC3C
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119137A3D-Cas9n-UGIBase editor made from APOBEC3D
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119138A3F-Cas9n-UGIBase editor made from APOBEC3F
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119139A3G-Cas9n-UGIBase editor made from APOBEC3G
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119140A3H HapI-Cas9n-UGIBase editor made from APOBEC3H Haplotype I
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119141A3H HapII-Cas9n-UGIBase editor made from APOBEC3H Haplotype II
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119142A3H HapII R175/6E-Cas9n-UGIBase editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE
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