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eGFP L138 gRNA
(Plasmid #119133)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119133 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MLM3636
  • Backbone manufacturer
    Joung Lab
  • Backbone size w/o insert (bp) 2278
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eGFP L138 gRNA
  • gRNA/shRNA sequence
    ccaacatttcagggcacaagc
  • Insert Size (bp)
    20
  • Mutation
    None
  • Promoter U6

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TTTCTTGGGTAGTTTGCAGTTTT
  • 3′ sequencing primer CGGTGCCACTTTTTCAAGTT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eGFP L138 gRNA was a gift from Reuben Harris (Addgene plasmid # 119133 ; http://n2t.net/addgene:119133 ; RRID:Addgene_119133)
  • For your References section:

    A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris RS. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. 10.1038/s41598-018-36739-9 PubMed 30679582