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pBS448 RSV-GFPcre
(Plasmid #11919)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 11919 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    bacteriophage P1
  • Insert Size (bp)
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBS448 carries a GFP-cre fusion gene under the control of the Rous sarcoma virus (RSV) LTR/promoter. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression. Higher level expression can be obtained using the EF1-alpha promoter plasmid pBS500 having the same structural fusion gene.

Use of the GFPcre fusion gene demonstrated that the Cre protein carries endogenous determinants that target the protein efficiently to the nucleus of eukaryotic cells. Thus the addition of an extraneous NLS is not required. The GFP-tagged cre gene is a convenient tool for directly observing the tissue-specificity of cre expression in transgenic mice.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS448 RSV-GFPcre was a gift from Brian Sauer (Addgene plasmid # 11919 ; ; RRID:Addgene_11919)
  • For your References section:

    Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Gagneten S, Le Y, Miller J, Sauer B. Nucleic Acids Res. 1997 Aug 15. 25(16):3326-31. 10.1093/nar/25.16.3326 PubMed 9241248