pGEX-4T1 14-3-3 epsilon K49E
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11945||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4969
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt name14-3-3 epsilon
SpeciesR. norvegicus (rat)
Entrez GeneYwhae (a.k.a. 14-3-3e)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SmaI (unknown if destroyed)
- 5′ sequencing primer pGEX_5
- 3′ sequencing primer pGEX_3 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-4T1 14-3-3 epsilon K49E was a gift from Michael Yaffe (Addgene plasmid # 11945 ; http://n2t.net/addgene:11945 ; RRID:Addgene_11945)
For your References section:14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport. Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV, Dalal SN, DeCaprio JA, Greenberg ME, Yaffe MB. J Cell Biol. 2002 Mar 4. 156(5):817-28. 10.1083/jcb.200112059 PubMed 11864996