pBS Sp1 full length
(Plasmid #12096)

• Depositing Lab: Robert Tjian
• Publication: Kadonaga et al Cell. 1987 Dec 24. 51(6):1079-90.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 3000
• Vector type: Cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sp1
• Species: H. sapiens (human)
• Insert Size (bp): 4400
• Entrez Gene: SP1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13pUC-Fwd [5'-CCCAGTCACGACGTTGTAAAACG-3']

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for more information.

This particular clone corresponds to the first 4237 nucleotides of GenBank reference sequence NM_138473.2 and contains the entire coding sequence of Sp1.