PurposeMultigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121092||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCas9 and hygromycin resist gene
- Promoter gpdA;trpC
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
a strong constitutive promoter -gpdA promoter to express fFuCas9, which is codon-optimized for efficient expression in Fusarium fujikuroi; a strong constitutive promoter -trpC promoter to express hygromycin resist gene for the selection
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC-fFuCas9-HTBNLS-hph was a gift from Xiao-Jun Ji (Addgene plasmid # 121092 ; http://n2t.net/addgene:121092 ; RRID:Addgene_121092)
For your References section:CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus Fusarium fujikuroi and Its Application in Strain Engineering for Gibberellic Acid Production. Shi TQ, Gao J, Wang WJ, Wang KF, Xu GQ, Huang H, Ji XJ. ACS Synth Biol. 2019 Feb 15;8(2):445-454. doi: 10.1021/acssynbio.8b00478. Epub 2019 Jan 23. 10.1021/acssynbio.8b00478 PubMed 30616338