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Addgene

pINDUCER20-hGfi1
(Plasmid #121200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pInducer20
  • Backbone size w/o insert (bp) 11794
  • Total vector size (bp) 12060
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Growth factor independent 1 transcriptional
  • Alt name
    Gfi1
  • Alt name
    ZNF163
  • Alt name
    SCN2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1266
  • Mutation
    P2L
  • GenBank ID
    NM_005263
  • Entrez Gene
    GFI1 (a.k.a. GFI-1, GFI1A, SCN2, ZNF163)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13/pUC Forward
  • 3′ sequencing primer M13/pUC Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a P2L mutation in the second amino acid of the plasmid insert. The depositing lab is aware of this and do not believe it affects the expression of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINDUCER20-hGfi1 was a gift from Noah Shroyer (Addgene plasmid # 121200 ; http://n2t.net/addgene:121200 ; RRID:Addgene_121200)

  • For your References section:

    Growth Factor-Independent 1 Is a Tumor Suppressor Gene in Colorectal Cancer. Chen MS, Lo YH, Chen X, Williams CS, Donnelly JM, Criss ZK 2nd, Patel S, Butkus JM, Dubrulle J, Finegold MJ, Shroyer NF. Mol Cancer Res. 2019 Mar;17(3):697-708. doi: 10.1158/1541-7786.MCR-18-0666. Epub 2019 Jan 3. 10.1158/1541-7786.MCR-18-0666 PubMed 30606770
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