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PCS2+ Cas9 Citations (6)

Originally described in: Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Gu B, Posfai E, Rossant J Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
PubMed Journal

Articles Citing PCS2+ Cas9

Articles
Efficient Generation of Large-Fragment Knock-In Mouse Models Using 2-Cell (2C)-Homologous Recombination (HR)-CRISPR. Gu B, Posfai E, Gertsenstein M, Rossant J. Curr Protoc Mouse Biol. 2020 Mar;10(1):e67. doi: 10.1002/cpmo.67. PubMed
Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach. Seleit A, Aulehla A, Paix A. Elife. 2021 Dec 6;10. pii: 75050. doi: 10.7554/eLife.75050. PubMed

Associated Plasmids

A novel ground truth dataset enables robust 3D nuclear instance segmentation in early mouse embryos. Nunley H, Shao B, Grover P, Singh J, Joyce B, Kim-Yip R, Kohrman A, Watters A, Gal Z, Kickuth A, Chalifoux M, Shvartsman S, Posfai E, Brown L. bioRxiv. 2023 Mar 15:2023.03.14.532646. doi: 10.1101/2023.03.14.532646. Preprint. PubMed
A Window into Mammalian Basement Membrane Development: Insights from the mTurq2-Col4a1 Mouse Model. Jones RA, Trejo B, Sil P, Little KA, Pasolli HA, Joyce B, Posfai E, Devenport D. bioRxiv [Preprint]. 2023 Sep 27:2023.09.27.559396. doi: 10.1101/2023.09.27.559396. PubMed
Nuclear instance segmentation and tracking for preimplantation mouse embryos. Nunley H, Shao B, Denberg D, Grover P, Singh J, Avdeeva M, Joyce B, Kim-Yip R, Kohrman A, Biswas A, Watters A, Gal Z, Kickuth A, Chalifoux M, Shvartsman SY, Brown LM, Posfai E. Development. 2024 Nov 1;151(21):dev202817. doi: 10.1242/dev.202817. Epub 2024 Nov 6. PubMed
Deletion of Xist repeat B disrupts cell cycle and asymmetric cell division through Usp9x hyperactivation in mice. Liang M, Zhang L, Gong H, Yang L, Wang H, Song N, Lai L, Xie W, Li Z. Nucleic Acids Res. 2025 Feb 27;53(5):gkaf142. doi: 10.1093/nar/gkaf142. PubMed

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