plenti2-EF1a-ChR2-EYFP-WRPE
(Plasmid #125255)

• Purpose: 3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation
• Depositing Lab: Roger D. Kamm
• Publication: Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi: 10.1126/sciadv.aat5847. eCollection 2018 Oct.

Backbone

• Vector backbone: pLenti-EF1a
• Total vector size (bp): 10523
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hChR2
• Alt name: Channelrhodopsin-2
• Species: H. sapiens (human)
• Insert Size (bp): 2354
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry