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plenti2-EF1a-ChR2-EYFP-WRPE
(Plasmid #125255)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 125255 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-EF1a
  • Total vector size (bp) 10523
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hChR2
  • Alt name
    Channelrhodopsin-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2354
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plenti2-EF1a-ChR2-EYFP-WRPE was a gift from Roger D. Kamm (Addgene plasmid # 125255 ; http://n2t.net/addgene:125255 ; RRID:Addgene_125255)
  • For your References section:

    Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons. Osaki T, Uzel SGM, Kamm RD. Sci Adv. 2018 Oct 10;4(10):eaat5847. doi: 10.1126/sciadv.aat5847. eCollection 2018 Oct. 10.1126/sciadv.aat5847 PubMed 30324134