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CROPseq-puro-v2 Citations (12)

Originally described in: Optical Pooled Screens in Human Cells.
Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity AJ, Zhang F, Blainey PC Cell. 2019 Oct 17;179(3):787-799.e17. doi: 10.1016/j.cell.2019.09.016.
PubMed Journal

Articles Citing CROPseq-puro-v2

Articles
The phenotypic landscape of essential human genes. Funk L, Su KC, Ly J, Feldman D, Singh A, Moodie B, Blainey PC, Cheeseman IM. Cell. 2022 Nov 23;185(24):4634-4653.e22. doi: 10.1016/j.cell.2022.10.017. Epub 2022 Nov 7. PubMed

Associated Plasmids

A genome-wide optical pooled screen reveals regulators of cellular antiviral responses. Carlson RJ, Leiken MD, Guna A, Hacohen N, Blainey PC. Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2210623120. doi: 10.1073/pnas.2210623120. Epub 2023 Apr 12. PubMed
CRISPRmap: Sequencing-free optical pooled screens mapping multi-omic phenotypes in cells and tissue. Gu J, Iyer A, Wesley B, Taglialatela A, Leuzzi G, Hangai S, Decker A, Gu R, Klickstein N, Shuai Y, Jankovic K, Parker-Burns L, Jin Y, Zhang JY, Hong J, Niu S, Chou J, Landau DA, Azizi E, Chan EM, Ciccia A, Gaublomme JT. bioRxiv [Preprint]. 2023 Dec 26:2023.12.26.572587. doi: 10.1101/2023.12.26.572587. PubMed
Sequential CRISPR screening reveals partial NatB inhibition as a strategy to mitigate alpha-synuclein levels in human neurons. Santhosh Kumar S, Naseri NN, Pather SR, Hallacli E, Ndayisaba A, Buenaventura C, Acosta K, Roof J, Fazelinia H, Spruce LA, Luk K, Khurana V, Rhoades E, Shalem O. Sci Adv. 2024 Feb 9;10(6):eadj4767. doi: 10.1126/sciadv.adj4767. Epub 2024 Feb 9. PubMed

Associated Plasmids

CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens. Walton RT, Qin Y, Blainey PC. bioRxiv [Preprint]. 2024 Mar 17:2024.03.17.585235. doi: 10.1101/2024.03.17.585235. PubMed
Single-cell image-based genetic screens systematically identify regulators of Ebola virus subcellular infection dynamics. Carlson RJ, Patten JJ, Stefanakis G, Soong BY, Radhakrishnan A, Singh A, Thakur N, Amarasinghe GK, Hacohen N, Basler CF, Leung D, Uhler C, Davey RA, Blainey PC. bioRxiv [Preprint]. 2024 Apr 7:2024.04.06.588168. doi: 10.1101/2024.04.06.588168. PubMed
Classification and functional characterization of regulators of intracellular STING trafficking identified by genome-wide optical pooled screening. Gentili M, Carlson RJ, Liu B, Hellier Q, Andrews J, Qin Y, Blainey PC, Hacohen N. bioRxiv [Preprint]. 2024 Apr 9:2024.04.07.588166. doi: 10.1101/2024.04.07.588166. PubMed
Classification and functional characterization of regulators of intracellular STING trafficking identified by genome-wide optical pooled screening. Gentili M, Carlson RJ, Liu B, Hellier Q, Andrews J, Qin Y, Blainey PC, Hacohen N. Cell Syst. 2024 Dec 18;15(12):1264-1277.e8. doi: 10.1016/j.cels.2024.11.004. Epub 2024 Dec 9. PubMed

Associated Plasmids

Optical Pooled Screening for the Discovery of Regulators of the Alternative Lengthening of Telomeres Pathway. Quintanilla I, Azeroglu B, Sagar MAK, Stracker TH, Denchi EL, Pegoraro G. bioRxiv [Preprint]. 2025 Feb 16:2025.02.15.638448. doi: 10.1101/2025.02.15.638448. PubMed
High-content image-based pooled screens reveal regulators of synaptogenesis. Le A, Biederer T, Blainey PC. Cell Rep. 2025 Jun 24;44(7):115889. doi: 10.1016/j.celrep.2025.115889. PubMed

Associated Plasmids

Single-cell image-based screens identify host regulators of Ebola virus infection dynamics. Carlson RJ, Patten JJ, Stefanakis G, Soong BY, Radhakrishnan A, Singh A, Thakur N, Sheehan KCF, Amarasinghe GK, Hacohen N, Basler CF, Leung DW, Uhler C, Davey RA, Blainey PC. Nat Microbiol. 2025 Aug;10(8):1989-2002. doi: 10.1038/s41564-025-02034-3. Epub 2025 Jul 24. PubMed
Temporal Clonal Tracing Reveals Tumor-Intrinsic IFNgamma-Dependencies Driving Niche Adaptation and Early Metastatic Colonization. Aleksandrovic E, Fross SR, Golomb SM, Liu X, Zhao Z, Das NM, Reese TC, Ma W, Lopez J, Zhong Z, Zhao M, Stack MS, Zhang S. bioRxiv [Preprint]. 2025 Oct 20:2025.08.13.669778. doi: 10.1101/2025.08.13.669778. PubMed

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