pGAPi
(Plasmid
#127547)
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Purpose(Empty Backbone) Gateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segment
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127547 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGAPi
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Backbone manufacturerVidali Lab
- Backbone size (bp) 11239
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Vector typeRNAi
- Promoter Ubiquitin
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Selectable markersHygromycin ; 2-Fluoroadenine
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer cttttgtcgatgctcaccctg
- 3′ sequencing primer ccggcaacaggattcaatctt (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGAPi was a gift from Luis Vidali (Addgene plasmid # 127547 ; http://n2t.net/addgene:127547 ; RRID:Addgene_127547) -
For your References section:
Robust survival-based RNAi of gene families using in tandem silencing of adenine phosphoribosyltransferase. Orr RG, Foley SJ, Sherman CA, Abreu I, Galotto G, Liu B, Gonzalez-Guerrero M, Vidali L. Plant Physiol. 2020 Aug 6. pii: pp.20.00865. doi: 10.1104/pp.20.00865. 10.1104/pp.20.00865 PubMed 32764132
Map uploaded by the depositor.
