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(Plasmid #127595)


Item Catalog # Description Quantity Price (USD)
Plasmid 127595 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Addgene plasmid # 39312
  • Backbone size w/o insert (bp) 6545
  • Total vector size (bp) 10266
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use BL21 bacterial cells for protein expression.
  • Copy number


  • Gene/Insert name
  • Species
    Bacterium from Ignavibacteriae phylum
  • Insert Size (bp)
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 6xHis Tag (N terminal on backbone)
    • Maltose binding protein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer acaacactagtgaaaacctgtatttccagggagcagcctcgATGAAAAAAGTATTAGGATTAGATCTTGGAGTATCTTCAAT
  • 3′ sequencing primer ttaaccggtatcaacttttcgtttctttttaggatcctcaacttttcgttttttcttaggtgacccTTTATTATTAGTAATAATTTCTCCCAAACAATTAATTAA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIgnaviCas9 was a gift from Stephen Quake (Addgene plasmid # 127595 ; ; RRID:Addgene_127595)
  • For your References section:

    Nucleic acid cleavage with a hyperthermophilic Cas9 from an uncultured Ignavibacterium. Schmidt ST, Yu FB, Blainey PC, May AP, Quake SR. Proc Natl Acad Sci U S A. 2019 Oct 28. pii: 1904273116. doi: 10.1073/pnas.1904273116. 10.1073/pnas.1904273116 PubMed 31659048