pNM58
(Plasmid #128409)

• Purpose: System with AIP and xylose inducible agrCA sensor
• Depositing Lab: Christopher Voigt
• Publication: Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 10.1038/s41589-019-0412-5.

Backbone

• Vector backbone: pXyl-AgrCA1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Need to grow cell in minimal medium (S750) with 1% arabinose. This plasmid doesn't work in LB.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: argCA
• Species: Staphylococcus aureus
• Promoter: PxylR

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gacttgtttaatcctgtaatctcagagagag
• 3′ sequencing primer: acttcgcagattattctatactgtgctaac

Gene/Insert 2

• Gene/Insert name: gfpmut2
• Species: Synthetic; Staphylococcus aureus
• Promoter: P3

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cctttaccttgtctacaaacccc
• 3′ sequencing primer: GCTGAAGTCAAGTTTGAAGGTGATACC

Resource Information

• Supplemental Documents:
  • pNM58.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry