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(Plasmid #129390)


Item Catalog # Description Quantity Price (USD)
Plasmid 129390 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Hoang TT
  • Backbone size w/o insert (bp) 5610
  • Total vector size (bp) 7656
  • Modifications to backbone
    Insertion of the rhamnose-inducible promoter system from Escherichia coli (derived from pSC201).
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Rhamnose-inducible promoter
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Promoter PrhaBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GTCAGCAATTTCGCCAGCAG
  • 3′ sequencing primer AAGTGGATCAGCAAGGACGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rhamnose-inducible promoter was originally found in E. coli. The system was cloned from a vector in the Cardona lab, known as pSC201.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAH-CTX1-rha was a gift from Silvia Cardona (Addgene plasmid # 129390 ; ; RRID:Addgene_129390)
  • For your References section:

    A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia. Hogan AM, Rahman ASMZ, Lightly TJ, Cardona ST. ACS Synth Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232. 10.1021/acssynbio.9b00232 PubMed 31491085