15x-QUAS-dTomato-T2A-GCaMP6s
(Plasmid #130666)

• Purpose: QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A
• Depositing Lab: Leslie Vosshall
• Publication: Matthews et al Elife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963.

Backbone

• Vector backbone: Addgene 104875, pBac-ECFP-15xQUAS_TATA-SV40
• Total vector size (bp): 8747
• Vector type: Insect Expression ; Ae. aegypti expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 15xQUAS-dTomato-T2A-GCaMP6s
• Insert Size (bp): 3469
• Promoter: 15xQUAS-TATA

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGCCAGGGTTTTCCCAGTCACGACG
• 3′ sequencing primer: GTCATAGCTGTTTCCTGT

Resource Information

• Supplemental Documents:
  • 15x-QUAS-dTomato-T2A-GCaMP6s.gb
• A portion of this plasmid was derived from a plasmid made by: Backbone is a gift from Chris Potter, Addgene #104875

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry