Addgene: The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti.

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Leslie Vosshall
Matthews et al

The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti.
Matthews BJ, Younger MA, Vosshall LB
Elife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
130665	Pub-mCD8:GFP-T2A-DsRed:NLS-SV40	Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti
130666	15x-QUAS-dTomato-T2A-GCaMP6s	QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A
130667	ppk301-T2A-QF2 HDR plasmid	Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

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