AAVS1 CAG rtTA3 TauWT 2N4R-EGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||132389||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8048
Vector typeMammalian Expression, Cre/Lox, CRISPR
Growth in Bacteria
Growth instructionsBacteria may truncate DNA, please select multiple clones and perform restriction digest after purification to confirm.
Copy numberHigh Copy
Gene/Insert namehuman TauWT 2N4R-EGFP
SpeciesH. sapiens (human), Synthetic
Entrez GeneMAPT (a.k.a. DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40)
- Promoter TREtight
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BbvCI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer TTCAGGTTGGACCGGTCCTCA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please note: Plasmid contains a 55 basepair deletion in the poly-GC track of the CAG promoter. It is unknown if this deletion affects plasmid function, but depositing lab was able to properly express proteins from the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAVS1 CAG rtTA3 TauWT 2N4R-EGFP was a gift from Gerold Schmitt-Ulms (Addgene plasmid # 132389 ; http://n2t.net/addgene:132389 ; RRID:Addgene_132389)
For your References section:Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Wang X, Friesen E, Müller I, Lemieux M, Dukart R, Maia IB, Kalia S, and Schmitt-Ulms G. Bio-protocol. 2020; 10(9): e3615 10.21769/BioProtoc.3615