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SiC-V2-Cas9G7
(Plasmid #133043)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133043 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tet repressor
  • gRNA/shRNA sequence
    Cas9G7
  • Tag / Fusion Protein
    • Cerulean

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dmitri Wiederschain (Addgene plasmid # 21915) and Sriram Neelamegham (Addgene plasmid # 64847)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SiC-V2-Cas9G7 was a gift from Sriram Neelamegham (Addgene plasmid # 133043 ; http://n2t.net/addgene:133043 ; RRID:Addgene_133043)
  • For your References section:

    Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing. Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski AB, Singhi N, Nemeth M, Neelamegham S. Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006. 10.1016/j.ymthe.2019.09.006 PubMed 31601489