pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
(Plasmid
#133560)
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Purpose(Empty Backbone) Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHD-3XP3-DsRed-DattP
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Backbone manufacturerIsaacman-Beck et al., 2019
- Backbone size (bp) 4081
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Modifications to backboneQ5 Site-Directed Mutagenesis used to remove most of the attP site,
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 was a gift from Tom Clandinin (Addgene plasmid # 133560 ; http://n2t.net/addgene:133560 ; RRID:Addgene_133560)