pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
(Plasmid #133560)

• Purpose: (Empty Backbone) Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus
• Depositing Lab: Tom Clandinin
• Publication: SPARC: a method to genetically manipulate precise proportions of cells. (unpublished)

Backbone

• Vector backbone: pHD-3XP3-DsRed-DattP
  (Search Vector Database)
• Backbone manufacturer: Isaacman-Beck et al., 2019
• Backbone size (bp): 4081
• Modifications to backbone: Q5 Site-Directed Mutagenesis used to remove most of the attP site,
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • pHD-3xP3-DsRed-CRISPR-donor{attP40}.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry