pCFD5-U6-3-t-attP40
(Plasmid #133561)

• Purpose: gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs
• Depositing Lab: Tom Clandinin
• Publication: SPARC: a method to genetically manipulate precise proportions of cells. (unpublished)

Backbone

• Vector backbone: pCFD5
  (Search Vector Database)
• Backbone manufacturer: Simon Bullock
• Backbone size w/o insert (bp): 6613
• Total vector size (bp): 6811
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: attP40 region guide RNAs
• gRNA/shRNA sequence: GTTACACAGCCCTTGGCTGG, CTGTGCTACCCAATGTGTGT
• Species: D. melanogaster (fly)
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACGTTTTATAACTTATGCCCCTAAG
• 3′ sequencing primer: GCACAATTGTCTAGAATGCATAC

Resource Information

• Supplemental Documents:
  • pCFD5-U6-3-t-attP40.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry