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Tom Clandinin Lab: SPARC: a method to genetically manipulate precise proportions of cells.
Isaacman-Beck J, Paik K, Wienecke C, Yang H, Fisher Y, Wang I, Ishida I, Maimon G, Wilson R, Clandinin T
bioRxiv
Article

Plasmids from Article

ID Plasmid Purpose  
133559pHD-3xP3-dsRed-DattPBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructs
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133560pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus
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133561pCFD5-U6-3-t-attP40gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs
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133562pHD-SPARC2-D-LexA::p65Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus
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133563pHD-SPARC2-I-LexA::p65Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus
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133564pHD-SPARC2-S-LexA::p65Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus
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133565pJFRC-20XUAS-IVS-PhiC31Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.
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133868pJFRC-nSyb-IVS-PhiC31Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.
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