SPARC: a method to genetically manipulate precise proportions of cells.
Isaacman-Beck J, Paik K, Wienecke C, Yang H, Fisher Y, Wang I, Ishida I, Maimon G, Wilson R, Clandinin T
bioRxiv Article
Isaacman-Beck J, Paik K, Wienecke C, Yang H, Fisher Y, Wang I, Ishida I, Maimon G, Wilson R, Clandinin T
bioRxiv Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 133559 | pHD-3xP3-dsRed-DattP | Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs |
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| 133560 | pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 | Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus |
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| 133561 | pCFD5-U6-3-t-attP40 | gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs |
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| 133562 | pHD-SPARC2-D-LexA::p65 | Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus |
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| 133563 | pHD-SPARC2-I-LexA::p65 | Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus |
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| 133564 | pHD-SPARC2-S-LexA::p65 | Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus |
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| 133565 | pJFRC-20XUAS-IVS-PhiC31 | Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest. |
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| 133868 | pJFRC-nSyb-IVS-PhiC31 | Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest. |
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