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pSiMPlh_N + pSiMPlh_C
(Plasmid #134316)


Item Catalog # Description Quantity Price (USD)
Plasmid 134316 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBAD33 + pTrc99a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
  • Alt name
  • Species
  • Mutation
    P254Q in hygR_gp41-1 N-intein

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).

Please see the Sequences link for the individual plasmid sequences.

The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSiMPlh_N + pSiMPlh_C was a gift from Barbara Di Ventura (Addgene plasmid # 134316 ; ; RRID:Addgene_134316)
  • For your References section:

    Split intein-mediated selection of cells containing two plasmids using a single antibiotic. Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B. Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1. 10.1038/s41467-019-12911-1 PubMed 31672972