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Addgene

SUPERBLADE5 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid SUPERBLADE5 with the following enzymes: EcoRI-HF and ScaI-HF.

Expected Results:
EcoRI-HF/ScaI-HF - 3362 bp, 1828 bp
EcoRI-HF - 5190 bp
ScaI-HF - 5190 bp

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Ladder and a digestion of the plasmid SUPERBLADE5 with the following enzymes: BsmBI

Expected bands:
BsmBI: Two bands: 3305 bp and 1885 bp

Results:
We observed the expected bands for this digest.