CS2 luc Fstl1
(Plasmid
#13497)
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Depositing Lab
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Depositing OrganizationFred Hutchinson Cancer Center
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 13497-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 13497-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+luc
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Backbone manufacturerSee Rupp et al (1994) Genes Dev 8:1311
- Backbone size w/o insert (bp) 5747
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFstl1
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Alt nameFollistatin-like-1
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SpeciesM. musculus (mouse)
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Insert Size (bp)147
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Mutationcontains 3 tandem copies of the following sequence from the 3'UTR of Fstl1: 5'-acactgagtaccagagggcaagcccacattcctcttctgctac-3'
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GenBank IDNM_008047
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Entrez GeneFstl1 (a.k.a. AI316791, AW107808, Fstl, TSC-36)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer 5'-cataaaggccaagaagggcg-3' (in luciferase near BglII site)
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for picture of empty pCS2-luc.
DNA (Catalog # 13497-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 13497-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CS2 luc Fstl1 was a gift from Stephen Tapscott (Addgene plasmid # 13497 ; http://n2t.net/addgene:13497 ; RRID:Addgene_13497) -
For your References section:
MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. Rosenberg MI, Georges SA, Asawachaicharn A, Analau E, Tapscott SJ. J Cell Biol. 2006 Oct 9. 175(1):77-85. 10.1083/jcb.200603039 PubMed 17030984
Map uploaded by the depositor.
