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pKT2-ACVR1-G328V-IRES-Katushka
(Plasmid #135002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKT2-IRES-Katushka
  • Backbone size w/o insert (bp) 4894
  • Total vector size (bp) 6424
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Activin A receptor, type I (ACVR1) G328V
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1530
  • Mutation
    c.983G>T, p.G328V
  • Entrez Gene
    ACVR1 (a.k.a. ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ACVR1 was modified from pCMV5-ALK2-WT (Addgene plasmid #11741).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT2-ACVR1-G328V-IRES-Katushka was a gift from Maria Castro (Addgene plasmid # 135002 ; http://n2t.net/addgene:135002 ; RRID:Addgene_135002)

  • For your References section:

    Therapeutic Efficacy of Immune Stimulatory Thymidine Kinase and fms-like Tyrosine Kinase 3 Ligand (TK/Flt3L) Gene Therapy in a Mouse Model of High-Grade Brainstem Glioma. Mendez F, Kadiyala P, Nunez FJ, Carney S, Nunez FM, Gauss JC, Ravindran R, Pawar S, Edwards M, Garcia-Fabiani MB, Haase S, Lowenstein PR, Castro MG. Clin Cancer Res. 2020 Aug 1;26(15):4080-4092. doi: 10.1158/1078-0432.CCR-19-3714. Epub 2020 Apr 24. 10.1158/1078-0432.CCR-19-3714 PubMed 32332014
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