pJMD14
(Plasmid
#138654)
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Purpose(Empty Backbone) phiOZJ integrating Streptomyces expression vector, intact RDF
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepJMD14
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Vector typeBacterial Expression
- Promoter PermE* variant sequence from pDA1652
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJMD14 was a gift from Joshua Blodgett (Addgene plasmid # 138654 ; http://n2t.net/addgene:138654 ; RRID:Addgene_138654) -
For your References section:
Construction of a new integrating vector from actinophage varphiOZJ and its use in multiplex Streptomyces transformation. Ko B, D'Alessandro J, Douangkeomany L, Stumpf S, deButts A, Blodgett J. J Ind Microbiol Biotechnol. 2019 Nov 8. pii: 10.1007/s10295-019-02246-7. doi: 10.1007/s10295-019-02246-7. 10.1007/s10295-019-02246-7 PubMed 31705217
