|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13918||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneModified pBluescript
- Backbone size (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.
pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.
For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSLAX13 (CT#232) was a gift from Cliff Tabin (Addgene plasmid # 13918 ; http://n2t.net/addgene:13918 ; RRID:Addgene_13918)
For your References section:Targeted gene misexpression in chick limb buds using avian replication-competent retroviruses. Logan M, Tabin C. Methods. 1998 Apr . 14(4):407-20. 10.1006/meth.1998.0595 PubMed 9608511
Map uploaded by the depositor.