RCAS(A) cTbx5 D1 (CT#636)
Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13968||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11600
Vector typeRetroviral ; avian expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesG. gallus (chicken)
MutationAmino acids 1-274
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Tbx5 D1 truncated (c-terminal truncation), putative dominant negative.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCAS(A) cTbx5 D1 (CT#636) was a gift from Cliff Tabin (Addgene plasmid # 13968 ; http://n2t.net/addgene:13968 ; RRID:Addgene_13968)
For your References section:Tbx5 is required for forelimb bud formation and continued outgrowth. Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP. Development. 2003 Jun . 130(12):2741-51. 10.1242/dev.00473 PubMed 12736217