Purpose(Empty Backbone) Base Cas12a CRISPRi plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||139750||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For CRISPR interference in Synechococcus sp. UTEX 2973 or other strains. Desired gRNA or gRNAs are inserted by AarI digestion of this plasmid followed by ligation.
Please not there are 3 mutations in RepC-E107D, C108A, H109M. Depositor confirms this does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSL3287 was a gift from Himadri Pakrasi (Addgene plasmid # 139750 ; http://n2t.net/addgene:139750 ; RRID:Addgene_139750)
For your References section:Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus sp. UTEX 2973. Knoot CJ, Biswas S, Pakrasi HB. ACS Synth Biol. 2020 Jan 17;9(1):132-143. doi: 10.1021/acssynbio.9b00417. Epub 2019 Dec 24. 10.1021/acssynbio.9b00417 PubMed 31829621