Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #139750)


Item Catalog # Description Quantity Price (USD)
Plasmid 139750 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For CRISPR interference in Synechococcus sp. UTEX 2973 or other strains. Desired gRNA or gRNAs are inserted by AarI digestion of this plasmid followed by ligation.

Please not there are 3 mutations in RepC-E107D, C108A, H109M. Depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL3287 was a gift from Himadri Pakrasi (Addgene plasmid # 139750 ; ; RRID:Addgene_139750)
  • For your References section:

    Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus sp. UTEX 2973. Knoot CJ, Biswas S, Pakrasi HB. ACS Synth Biol. 2020 Jan 17;9(1):132-143. doi: 10.1021/acssynbio.9b00417. Epub 2019 Dec 24. 10.1021/acssynbio.9b00417 PubMed 31829621