hsp70l: hAPOB48-mCherry.
(Plasmid #140639)

• Purpose: Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.
• Depositing Lab: Steven Farber
• Publication: Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119000259. doi: 10.1194/jlr.RA119000259.

Backbone

• Vector backbone: pDEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3156
• Total vector size (bp): 13397
• Vector type: zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: hsp70l
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1566
• Entrez Gene: hsp70l (a.k.a. HSP70, hsp70-4)
• Promoter: hsp70l

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: APOB
• Species: H. sapiens (human)
• Mutation: APOB48: amino acid 1-2180
• Entrez Gene: APOB (a.k.a. FCHL2, FLDB, LDLCQ4, apoB-100, apoB-48)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GGG GAC AAG TTT GTA CAA AAA AGC AGG CTC AAT GGA CCC GCC GAG GCC C
• 3′ sequencing primer: GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC TTG TAT CAT ATA TGT CTG CAG TTG

Resource Information

• Supplemental Documents:
  • hsp70-apob48-mcherry-mod.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry