pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro Citations (13)
Originally described in: A SARS-CoV-2 protein interaction map reveals targets for drug repurposing.Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. PubMed Journal
Articles Citing pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro
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| Genes with 5' terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 Nsp1 protein. Rao S, Hoskins I, Tonn T, Garcia PD, Ozadam H, Sarinay Cenik E, Cenik C. RNA. 2021 Sep;27(9):1025-1045. doi: 10.1261/rna.078661.120. Epub 2021 Jun 14. PubMed |
| SARS-CoV-2 viral proteins NSP1 and NSP13 inhibit interferon activation through distinct mechanisms. Vazquez C, Swanson SE, Negatu SG, Dittmar M, Miller J, Ramage HR, Cherry S, Jurado KA. PLoS One. 2021 Jun 24;16(6):e0253089. doi: 10.1371/journal.pone.0253089. eCollection 2021. PubMed |
| Effect of SARS-CoV-2 proteins on vascular permeability. Rauti R, Shahoha M, Leichtmann-Bardoogo Y, Nasser R, Paz E, Tamir R, Miller V, Babich T, Shaked K, Ehrlich A, Ioannidis K, Nahmias Y, Sharan R, Ashery U, Maoz BM. Elife. 2021 Oct 25;10. pii: 69314. doi: 10.7554/eLife.69314. PubMed |
| SARS-CoV-2 inhibits induction of the MHC class I pathway by targeting the STAT1-IRF1-NLRC5 axis. Yoo JS, Sasaki M, Cho SX, Kasuga Y, Zhu B, Ouda R, Orba Y, de Figueiredo P, Sawa H, Kobayashi KS. Nat Commun. 2021 Nov 15;12(1):6602. doi: 10.1038/s41467-021-26910-8. PubMed |
| COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling. Sansico F, Miroballo M, Bianco DS, Tamiro F, Colucci M, Santis E, Rossi G, Rosati J, Di Mauro L, Miscio G, Mazza T, Vescovi AL, Mazzoccoli G, Giambra V, On Behalf Of Css-Covid Group. Biomedicines. 2021 Nov 29;9(12). pii: biomedicines9121794. doi: 10.3390/biomedicines9121794. PubMed |
| Cyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral spread. Gupta RK, Mlcochova P. EMBO J. 2022 Nov 17;41(22):e111653. doi: 10.15252/embj.2022111653. Epub 2022 Oct 10. PubMed |
| SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D. Lee MJ, Leong MW, Rustagi A, Beck A, Zeng L, Holmes S, Qi LS, Blish CA. Cell Rep. 2022 Dec 27;41(13):111892. doi: 10.1016/j.celrep.2022.111892. Epub 2022 Dec 12. PubMed |
| Secreted ORF8 induces monocytic pro-inflammatory cytokines through NLRP3 pathways in patients with severe COVID-19. Wu X, Manske MK, Ruan GJ, Witter TL, Nowakowski KE, Abeykoon JP, Tang X, Yu Y, Gwin KA, Wu A, Taupin V, Bhardwaj V, Paludo J, Dasari S, Dong H, Ansell SM, Badley AD, Schellenberg MJ, Witzig TE. iScience. 2023 Jun 16;26(6):106929. doi: 10.1016/j.isci.2023.106929. Epub 2023 May 18. PubMed |
| Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53. Lee JD, Menasche BL, Mavrikaki M, Uyemura MM, Hong SM, Kozlova N, Wei J, Alfajaro MM, Filler RB, Muller A, Saxena T, Posey RR, Cheung P, Muranen T, Heng YJ, Paulo JA, Wilen CB, Slack FJ. Cell Rep. 2023 Dec 26;42(12):113478. doi: 10.1016/j.celrep.2023.113478. Epub 2023 Nov 21. PubMed |
| Evasion of NKG2D-mediated cytotoxic immunity by sarbecoviruses. Hartmann JA, Cardoso MR, Talarico MCR, Kenney DJ, Leone MR, Reese DC, Turcinovic J, O'Connell AK, Gertje HP, Marino C, Ojeda PE, De Paula EV, Orsi FA, Velloso LA, Cafiero TR, Connor JH, Ploss A, Hoelzemer A, Carrington M, Barczak AK, Crossland NA, Douam F, Boucau J, Garcia-Beltran WF. Cell. 2024 May 9;187(10):2393-2410.e14. doi: 10.1016/j.cell.2024.03.026. Epub 2024 Apr 22. PubMed |
| SARS-CoV-2 Accessory Protein ORF8 Targets the Dimeric IgA Receptor pIgR. Laprise F, Arduini A, Duguay M, Pan Q, Liang C. Viruses. 2024 Jun 22;16(7):1008. doi: 10.3390/v16071008. PubMed |
Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.
Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M.
Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
PubMed
Associated Plasmids |
| Ribo-ITP expands the translatome of limited input samples. Ghatpande V, Paul U, Howard MA, Cenik C. bioRxiv [Preprint]. 2025 Aug 4:2025.08.04.668486. doi: 10.1101/2025.08.04.668486. PubMed |
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