pRD119 (PCNA GFP)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14822||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. melanogaster (fly)
Entrez GenePCNA (a.k.a. Dmel_CG9193, 53/13, CG9193, DmPCNA, DmPCNA1, Dmel\CG9193, MUS209, Mus209, PCNA/mus209, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, mus 209, mus209, pcna)
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
PCNA GFP fusion (glow); Asp718-NotI fragment from pRD113 cloned into pCasper4IN; GFP fused in-frame using 5' blunt NheI and 3' XbaI (replaces PCNA coding to STOP [XbaI] but has PCNA 3'; GFP from pNEGFPX.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRD119 (PCNA GFP) was a gift from Robert Duronio (Addgene plasmid # 14822 ; http://n2t.net/addgene:14822 ; RRID:Addgene_14822)
For your References section:The contribution of E2F-regulated transcription to Drosophila PCNA gene function. Thacker SA, Bonnette PC, Duronio RJ. Curr Biol. 2003 Jan 8. 13(1):53-8. 10.1016/S0960-9822(02)01400-8 PubMed 12526745
Map uploaded by the depositor.