pRD119 (PCNA GFP)
(Plasmid #14822)

• Depositing Lab: Robert Duronio
• Publication: Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

Backbone

• Vector backbone: na
• Backbone manufacturer: na
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCNA-GFP
• Alt name: PCNA
• Species: D. melanogaster (fly)
• Entrez Gene: PCNA (a.k.a. Dmel_CG9193, 53/13, CG9193, DmPCNA, DmPCNA1, Dmel\CG9193, MUS209, Mus209, PCNA/mus209, PCNA1, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, mus 209, mus209, pcna)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCNA GFP fusion (glow); Asp718-NotI fragment from pRD113 cloned into pCasper4IN; GFP fused in-frame using 5' blunt NheI and 3' XbaI (replaces PCNA coding to STOP [XbaI] but has PCNA 3'; GFP from pNEGFPX.1.