pRosa mNotch1 (DM#273)
(Plasmid
#15066)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15066 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRosa26PAm1
- Backbone size w/o insert (bp) 9882
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Vector typeCre/Lox ; for making transgenic mice
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNotch
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Alt nameNotch1
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SpeciesM. musculus (mouse)
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Insert Size (bp)6339
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Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
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Tags
/ Fusion Proteins
- PGK-Neomycin (N terminal on insert)
- IRES-EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byNotch provided by Sean Megason and Andy McMahon
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Targeting construct used to make Rosa-Notch knock-in mice.
Encodes (from 5' to 3') an intracellular fragment of mouse Notch1 (amino acids 1749–2293, lacking the C-terminal PEST domain), an internal ribosome entry sequence, and nuclear-localized enhanced GFP (EGFP).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRosa mNotch1 (DM#273) was a gift from Douglas Melton (Addgene plasmid # 15066) -
For your References section:
Notch signaling controls multiple steps of pancreatic differentiation. Murtaugh LC, Stanger BZ, Kwan KM, Melton DA. Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5. 10.1073/pnas.2436557100 PubMed 14657333
Map uploaded by the depositor.