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pRosa mNotch1 (DM#273)
(Plasmid #15066)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15066 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRosa26PAm1
  • Backbone size w/o insert (bp) 9882
  • Vector type
    Cre/Lox ; for making transgenic mice
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Notch
  • Alt name
    Notch1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6339
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
  • Tags / Fusion Proteins
    • PGK-Neomycin (N terminal on insert)
    • IRES-EGFP (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Notch provided by Sean Megason and Andy McMahon

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Targeting construct used to make Rosa-Notch knock-in mice.

Encodes (from 5' to 3') an intracellular fragment of mouse Notch1 (amino acids 1749–2293, lacking the C-terminal PEST domain), an internal ribosome entry sequence, and nuclear-localized enhanced GFP (EGFP).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRosa mNotch1 (DM#273) was a gift from Douglas Melton (Addgene plasmid # 15066)
  • For your References section:

    Notch signaling controls multiple steps of pancreatic differentiation. Murtaugh LC, Stanger BZ, Kwan KM, Melton DA. Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5. 10.1073/pnas.2436557100 PubMed 14657333