vPyACR_21821
(Plasmid #153030)

• Purpose: Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
• Depositing Lab: Peter Hegemann
• Publication: Rozenberg et al Current Biology. 2020. 30: 1–11

Backbone

• Vector backbone: pEGFP-N1
  (Search Vector Database)
• Backbone size w/o insert (bp): 3980
• Total vector size (bp): 5758
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: vPyACR_21821
• Species: Synthetic; Marine Giant Virus
• Insert Size (bp): 834
• Mutation: vPyACR_21821 was C-terminally truncated to include only the transmembrane region. eYFP is flanked by a membrane trafficking signal and an ER release signal N- and C-terminally, respectively.
• GenBank ID: MT353683
• Promoter: CMV (+ enhancer)
• Tag / Fusion Protein:
  • eYFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-fwd: GCAAATGGGCGGTAGGCGT or EGFP-N1-fwd: GAGGTCTATATAAGCAGAGC
• 3′ sequencing primer: EGFP-C1-rev: AACCATTATAAGCTGCAATAAAC

Resource Information

• Supplemental Documents:
  • vPyACR_21821_eYFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry