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Py2087ACR1
(Plasmid #153032)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153032 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 3986
  • Total vector size (bp) 6278
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Py2087ACR1
  • Species
    Pyramimonas spec.
  • Insert Size (bp)
    1575
  • GenBank ID
    MT353681
  • Promoter CMV (+ enhancer)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BshTI (not destroyed)
  • 5′ sequencing primer CMV-fwd: GCAAATGGGCGGTAGGCGT or EGFP-N1-fwd: GAGGTCTATATAAGCAGAGC
  • 3′ sequencing primer EGFP-C1-rev: AACCATTATAAGCTGCAATAAAC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Py2087ACR1 was a gift from Peter Hegemann (Addgene plasmid # 153032 ; http://n2t.net/addgene:153032 ; RRID:Addgene_153032)
  • For your References section:

    Lateral Gene Transfer of Anion-Conducting Channelrhodopsins between Green Algae and Giant Viruses. Rozenberg A, Oppermann J, Wietek J, Lahore RGF, Sandaa RA, Bratbak G, Hegemann P, Béjà O. Current Biology. 2020. 30: 1–11 10.1016/j.cub.2020.09.056