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pcDNA5-FRT-TO-Nprot-HF_GC3opt
(Plasmid #157732)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 157732 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    pcDNA5-FRT-TO
  • Backbone manufacturer
    Thermo Fisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nprot-HF_GC3opt
  • Alt name
    SARS-CoV-2 N (nucleocapsid)
  • Species
    SARS-CoV-2
  • Mutation
    codon optimized; gene expression was optimized by increasing the GC3 content within codons
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Tag / Fusion Protein
    • His8-Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene Art

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-TO-Nprot-HF_GC3opt was a gift from David Tollervey (Addgene plasmid # 157732 ; http://n2t.net/addgene:157732 ; RRID:Addgene_157732)