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PurposeA S. pneumoniae not integrated, temperature sensitive plasmid that carries the CRISPR system; a Zn inducible wtCas9 and a sgRNA which has its spacer sequence replaced by gfp gene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcdf1-b
- Backbone size w/o insert (bp) 1718
- Total vector size (bp) 9358
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Vector typeBacterial Expression, CRISPR
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Selectable markerserythromycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namewtCas9
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SpeciesS. pyogenes
Gene/Insert 2
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Gene/Insert namegfp
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Insert Size (bp)738
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2020.06.13.149682 for bioRxiv preprint.
To obtain this plasmid, please reach out directly to the depositing laboratory.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDS05 was a gift from Jan-Willem Veening (Addgene plasmid # 157913) -
For your References section:
Harnessing CRISPR-Cas9 for Genome Editing in Streptococcus pneumoniae D39V. Synefiaridou D, Veening JW. Appl Environ Microbiol. 2021 Feb 26;87(6). pii: AEM.02762-20. doi: 10.1128/AEM.02762-20. Print 2021 Feb 26. 10.1128/AEM.02762-20 PubMed 33397704
Map uploaded by the depositor.
