Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed
(Plasmid
#157974)
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PurposeTemplate plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepsL1180
- Total vector size (bp) 3422
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOrco
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SpeciesAedes aegypti
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Insert Size (bp)18
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GenBank IDNM_001358471
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CTCGTTCAACTCAAGGAT
- 3′ sequencing primer CTCGTTCAACTCAAGGAT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed was a gift from Carolyn McBride (Addgene plasmid # 157974 ; http://n2t.net/addgene:157974 ; RRID:Addgene_157974) -
For your References section:
Mosquito brains encode unique features of human odour to drive host seeking. Zhao Z, Zung JL, Hinze A, Kriete AL, Iqbal A, Younger MA, Matthews BJ, Merhof D, Thiberge S, Ignell R, Strauch M, McBride CS. Nature. 2022 May;605(7911):706-712. doi: 10.1038/s41586-022-04675-4. Epub 2022 May 4. 10.1038/s41586-022-04675-4 PubMed 35508661
Map uploaded by the depositor.
