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(Plasmid #47917)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47917 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 5066
  • Total vector size (bp) 5825
  • Modifications to backbone
    Includes Aedes aegypti polyubiquitin (PUb) promoter and SV40 polyadenylation sequence.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Enhanced Cyan Fluorescent Protein
  • Species
    Aequorea victoria
  • Insert Size (bp)
  • Promoter Aedes aegypti polyubiquitin (PUb)
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13_reverse_primer
  • 3′ sequencing primer M13_forward20_primer
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Aedes aegypti PUb promoter used in pSL1180-HR-PUbECFP was PCR-amplified from pMos-3xP3DsRed-PUb-EGFP-attP (a kind gift of Dr Zach Adelman, Virginia Tech). Anderson et al. (2010) Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti. Insect Mol Biol. 19:441-9.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Dr Conor McMeniman, Vosshall lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL1180-HR-PUbECFP was a gift from Leslie Vosshall (Addgene plasmid # 47917 ; ; RRID:Addgene_47917)