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PSL1180polyUBdsRED
(Plasmid #49327)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49327 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    psl1180
  • Backbone size w/o insert (bp) 3422
  • Total vector size (bp) 5574
  • Modifications to backbone
    Aedes polyubiquitin promoter and dsRED were cloned as a single fragment into psl1180 using BamH1 and Mlu1.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dsRED
  • Insert Size (bp)
    681
  • Promoter Aedes polyubiquitin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Mlu1 (not destroyed)
  • 5′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Aedes aegypti PUb promoter and dsRED used in pSL1180polyUBdsRED was cloned using BamH1/Mlu1 from pMos-PUbDsRed-5HE-MCS-5HE as cited in Carpenetti et al. (2012) Insect Mol Biol. 21 : 97-106 (a kind gift of Dr Zach Adelman, Virginia Tech).
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

Addgene's quality control sequencing finds a one nucleotide discrepancy in the Aedes aegypti PUb promoter. This discrepancy does not affect promoter function, and the plasmid should work as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PSL1180polyUBdsRED was a gift from Leslie Vosshall (Addgene plasmid # 49327)