pLPCX roGFP2-Prx2(C172A)
(Plasmid
#158228)
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PurposeGenetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLpcX
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameroGFP2-Prx2(C172A)
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPCX roGFP2-Prx2(C172A) was a gift from Tobias Dick (Addgene plasmid # 158228 ; http://n2t.net/addgene:158228 ; RRID:Addgene_158228) -
For your References section:
Real-time monitoring of peroxiredoxin oligomerization dynamics in living cells. Pastor-Flores D, Talwar D, Pedre B, Dick TP. Proc Natl Acad Sci U S A. 2020 Jun 29. pii: 1915275117. doi: 10.1073/pnas.1915275117. 10.1073/pnas.1915275117 PubMed 32601209
