pT7-SpCas9_sgRNA-site1 (RTW443) Citations (2)
Originally described in: Scalable characterization of the PAM requirements of CRISPR-Cas enzymes using HT-PAMDA.Walton RT, Hsu JY, Joung JK, Kleinstiver BP Nat Protoc. 2021 Feb 5. pii: 10.1038/s41596-020-00465-2. doi: 10.1038/s41596-020-00465-2. PubMed Journal
Articles Citing pT7-SpCas9_sgRNA-site1 (RTW443)
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PAM-flexible genome editing with an engineered chimeric Cas9.
Zhao L, Koseki SRT, Silverstein RA, Amrani N, Peng C, Kramme C, Savic N, Pacesa M, Rodriguez TC, Stan T, Tysinger E, Hong L, Yudistyra V, Ponnapati MR, Jacobson JM, Church GM, Jakimo N, Truant R, Jinek M, Kleinstiver BP, Sontheimer EJ, Chatterjee P.
Nat Commun. 2023 Oct 4;14(1):6175. doi: 10.1038/s41467-023-41829-y.
PubMed
Associated Plasmids |
Custom CRISPR-Cas9 PAM variants via scalable engineering and machine learning.
Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP.
Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
PubMed
Associated Plasmids |
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