pCTCON2-GYB
(Plasmid #160927)

• Purpose: Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)
• Depositing Lab: James Van Deventer
• Publication: Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-588. doi: 10.1039/c9me00107g. Epub 2020 Jan 23.

Backbone

• Vector backbone: pCTCON2
• Backbone size w/o insert (bp): 6314
• Total vector size (bp): 7193
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: BFP
• Insert Size (bp): 699
• Promoter: GAL1-10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGCATAACCACTTTAACTAATACTTTC
• 3′ sequencing primer: GTAAAGTTGGTAACGGAACG

Gene/Insert 2

• Gene/Insert name: sfGFP
• Insert Size (bp): 720
• Promoter: GAL1-10

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGCATAACCACTTTAACTAATACTTTC
• 3′ sequencing primer: GTAAAGTTGGTAACGGAACG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The GFP gene was originally amplified from pRYG, obtained from the laboratory of Jeffrey Barrick at The University of Texas Austin (Addgene plasmid #113642). The BFP gene was originally amplified from pBAD-mTagBFP2, obtained from the laboratory of Nikhil U. Nair at Tufts University and originally purchased from Addgene (Addgene plasmid #34632)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry